FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596
Total Bases231.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG46629.19799498746867No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC30318.984962406015036No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC26416.541353383458645No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT19612.280701754385964No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC1106.8922305764411025No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT130.8145363408521302No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC110.6892230576441103No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG80.5012531328320802No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTATAGGGGCACTCAGA70.43859649122807015No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.37593984962406013No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT60.37593984962406013No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT50.3132832080200501No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGCCCAAGTCTC50.3132832080200501No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC50.3132832080200501No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT40.2506265664160401No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT30.18796992481203006No Hit
CCTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC30.18796992481203006No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.18796992481203006No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAA30.18796992481203006No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT30.18796992481203006No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT30.18796992481203006No Hit
ACTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT30.18796992481203006No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCCGCCTC30.18796992481203006No Hit
TGGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.18796992481203006No Hit
ATTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT30.18796992481203006No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCTT30.18796992481203006No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT30.18796992481203006No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT20.12531328320802004No Hit
TGTGTTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.12531328320802004No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTCCCTC20.12531328320802004No Hit
ACTTAAAAACAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTCT20.12531328320802004No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.12531328320802004No Hit
ACTTAAAAACAAGGTAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC20.12531328320802004No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.12531328320802004No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT20.12531328320802004No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACAAAGTCTC20.12531328320802004No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT20.12531328320802004No Hit
TGTGTGCGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG20.12531328320802004No Hit
ACTTAAAAACAAGGCAGTGCTAGTGCTAATGGTGCTACAGTTTCTGCCTC20.12531328320802004No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.12531328320802004No Hit
TGCGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG20.12531328320802004No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTTTGGACAGACCAAGTCTCT20.12531328320802004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)