FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4108
Total Bases595.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC100024.342745861733203No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC74718.184031158714703No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG62215.141187925998054No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC56513.753651411879261No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1684.089581304771178No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG551.338851022395326No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT551.338851022395326No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT300.7302823758519961No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT250.6085686465433301No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.4381694255111977No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.31645569620253167No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.26777020447906524No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT110.26777020447906524No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.24342745861733206No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.24342745861733206No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG100.24342745861733206No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT100.24342745861733206No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.21908471275559885No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.19474196689386564No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.17039922103213243No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.17039922103213243No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.17039922103213243No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGTAAATTGCTGCTGGAACC60.1460564751703992No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1460564751703992No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.1460564751703992No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC60.1460564751703992No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.1460564751703992No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1460564751703992No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC60.1460564751703992No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC60.1460564751703992No Hit
AAATCCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.12171372930866603No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTTGAACC50.12171372930866603No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.12171372930866603No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.12171372930866603No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC50.12171372930866603No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.12171372930866603No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA50.12171372930866603No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTACATTATTTCAATTAAATAC50.12171372930866603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)