FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14734
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC466031.62752816614633No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC324222.00352925207004No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG180012.216641780914891No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC13789.352517985611511No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG4963.3663635129632143No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1380.936609203203475No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA750.5090267408714538No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT690.4683046016017375No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA610.4140084159087824No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.35292520700420793No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT420.2850549748880141No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT410.27826795167639473No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT380.25790688204153656No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG360.24433283561829783No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.24433283561829783No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.24433283561829783No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.23075878919505904No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.23075878919505904No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.23075878919505904No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT330.22397176598343968No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.21718474277182026No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.2103977195602009No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.19682367313696214No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.19682367313696214No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.18324962671372336No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC250.1696755802904846No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.15610153386724585No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG220.14931451065562645No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.14931451065562645No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.14931451065562645No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.14252748744400706No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC200.13574046423238767No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT190.12895344102076828No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.12216641780914891No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.11537939459752952No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA160.10859237138591013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)