FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5091
Total Bases738 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC173634.0993910823021No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG105620.74248674130819No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC84616.61756040070713No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2484.871341583186014No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG651.2767629149479474No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT651.2767629149479474No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT400.7857002553525829No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT240.4714201532115498No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT210.41249263406010606No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT200.39285012767629146No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT200.39285012767629146No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.3339226085248478No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAGATTATTTCAATTAAATAC160.3142801021410332No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG140.27499508937340406No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT130.25535258298958946No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT120.2357100766057749No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.2357100766057749No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.19642506383814573No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.1767825574543312No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC90.1767825574543312No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.1571400510705166No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.1571400510705166No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT80.1571400510705166No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC70.13749754468670203No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC70.13749754468670203No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.13749754468670203No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC70.13749754468670203No Hit
AAATCCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.11785503830288745No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.11785503830288745No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC60.11785503830288745No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGTTTGCAAATTGCTGCTGGAACC60.11785503830288745No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.11785503830288745No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.11785503830288745No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.11785503830288745No Hit
AAATACCGTTCAGAGCTGCAACCAGGAGCTTGCAAATTGCTGCTGGAACC60.11785503830288745No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTTCTGCTGGAACC60.11785503830288745No Hit
AAATACCGTTCAGAGTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.11785503830288745No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC60.11785503830288745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)