FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7720
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC244231.63212435233161No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC191424.792746113989637No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG115915.012953367875648No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2663.44559585492228No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1361.7616580310880827No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG851.1010362694300517No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT720.932642487046632No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT500.6476683937823834No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGAAAGCCAATGGGAAA400.5181347150259068No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA380.4922279792746114No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT360.466321243523316No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.3626943005181347No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.3626943005181347No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.34974093264248707No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.3238341968911917No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG230.2979274611398964No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.2979274611398964No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.2849740932642487No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.27202072538860106No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.22020725388601034No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.20725388601036268No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.20725388601036268No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC140.18134715025906736No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA130.1683937823834197No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.15544041450777202No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.15544041450777202No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.15544041450777202No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.14248704663212436No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATGGAGAGGCCCA110.14248704663212436No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.14248704663212436No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.1295336787564767No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT100.1295336787564767No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.1295336787564767No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.1295336787564767No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC100.1295336787564767No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAAATAAATAC80.10362694300518134No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT80.10362694300518134No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTACAAATTGCTGCTGGAACC80.10362694300518134No Hit
GGCACTAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACAA80.10362694300518134No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGTTGGAACC80.10362694300518134No Hit
AAATACCGTTCAGAGCTGCCGCCAGGAGCTTGCAAATTGCTGCTGGAACC80.10362694300518134No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)