FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8661
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC414847.89285301928184No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG158918.34661124581457No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6737.770465304237385No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG4134.768502482392333No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG891.027594965939268No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA690.796674748874264No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA540.6234845860755109No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA470.5426625101027596No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA450.5195704883962591No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT350.4041103798637571No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.38101835815725665No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT330.38101835815725665No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.36947234730400647No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.3579263364507563No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.3579263364507563No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.26555824962475466No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG220.2540122387715045No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT190.21937420621175382No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.21937420621175382No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA170.19628218450525345No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT160.18473617365200323No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT160.18473617365200323No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT140.16164415194550283No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC120.13855213023900242No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC110.12700611938575224No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.12700611938575224No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG100.11546010853250202No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTAAATTATTTCAATTAAATAC100.11546010853250202No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT100.11546010853250202No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTCCAATTAAATAC100.11546010853250202No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTAAATTATTTCAATTAAATAC100.11546010853250202No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT100.11546010853250202No Hit
GGCACTAGAATGGACCTCCTCCTCATCACTAAATTATTTCAATTAAATAC90.10391409767925182No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATCATTTCAATTAAATAC90.10391409767925182No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC90.10391409767925182No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC90.10391409767925182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)