FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7321
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC358648.98237945635842No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC108014.752083048763831No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG79710.886490916541456No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2052.800163912033875No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA680.9288348586258708No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA450.6146701270318262No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA420.5736921185630378No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG410.560032782406775No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA410.560032782406775No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA400.5463734462505122No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA350.4780767654691982No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT340.4644174293129354No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.43709875700040973No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.4097800846878842No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.3961207485316214No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT220.30050539543778176No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT220.30050539543778176No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG180.2458680508127305No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC140.1912307061876793No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC130.17757137003141646No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT130.17757137003141646No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.16391203387515366No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.15025269771889088No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGAAAGCCAATGGGAAA110.15025269771889088No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.15025269771889088No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT110.15025269771889088No Hit
GGCACTAGAATGGACCTCTTCCTCGTCACTAAATTATTTCAATTAAATAC100.13659336156262805No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC100.13659336156262805No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC90.12293402540636525No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.12293402540636525No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTAAATTATTTCAATTAAATAC90.12293402540636525No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTAAATTATTTCAATTAAATAC80.10927468925010243No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.10927468925010243No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA80.10927468925010243No Hit
GGCACTAGAATGGACCTCTTACTCATCACTAAATTATTTCAATTAAATAC80.10927468925010243No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)