FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035
Total Bases150 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC64462.22222222222222No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC17116.52173913043478No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC292.8019323671497585No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT191.8357487922705313No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT111.0628019323671498No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT90.8695652173913043No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTGGACAGACCAAGTCTCT80.7729468599033816No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT80.7729468599033816No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTTTGGACAGACCAAGTCTCT60.5797101449275363No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT50.4830917874396135No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT50.4830917874396135No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT50.4830917874396135No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTTTGGACAGACCAAGTCTCT50.4830917874396135No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT40.3864734299516908No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT40.3864734299516908No Hit
ACTTAAAAACAAGGCAGTTCTGAACGTATTTTTGGACAGACCAAGTCTCT30.2898550724637681No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT30.2898550724637681No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACAAAGTCTC30.2898550724637681No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGCCCAAGTCTC30.2898550724637681No Hit
ACTTAAAAAAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT30.2898550724637681No Hit
ACTTAAAAACAAGGCAGCGCTGAACGGTATTTTTGGACAGACCAAGTCTC30.2898550724637681No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGGTCCATTCTAGTGCCTT20.1932367149758454No Hit
ACTTAAAAACAAGGCAGCTCTGAACGCTATTTTTGGACAGACCAAGTCTC20.1932367149758454No Hit
ACTTAAAAACAAGGCAGCTCCGAACGGTATTTTTGGACAGACCAAGTCTC20.1932367149758454No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT20.1932367149758454No Hit
ACTTAAAAACAAGGCAGTGCTGAACGATATTTTTGGACAGACCAAGTCTC20.1932367149758454No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGTACATGG20.1932367149758454Clontech Universal Primer Mix Long (96% over 27bp)
ACTTAAAAACAAGGCAGTTCTAACGGTATTTTTGGACAGACCAAGTCTCT20.1932367149758454No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.1932367149758454Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGCTCTGAACGATATTTTTGGACAGACCAAGTCTC20.1932367149758454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)