FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035
Total Bases150 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG63761.54589371980676No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG16816.231884057971012No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG272.608695652173913No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG191.8357487922705313No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT90.8695652173913043No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT90.8695652173913043No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG80.7729468599033816No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT80.7729468599033816No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC70.6763285024154589Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT50.4830917874396135No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.4830917874396135No Hit
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.4830917874396135No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.4830917874396135No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.3864734299516908No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT40.3864734299516908No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATGGCTAATGTAGAACCATGT30.2898550724637681No Hit
AAATACCGTTCAGAGCTGCCTTTTTTTAAGTGGCTAATGTAGAACCATGT30.2898550724637681No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.2898550724637681No Hit
AAATACCGTTCAGCGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG30.2898550724637681No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.1932367149758454No Hit
AAATACCGTTCGGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG20.1932367149758454No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.1932367149758454No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGATAATGTAGAACCATG20.1932367149758454No Hit
AAATACGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.1932367149758454No Hit
GGCACTAGAATGGACCTCTGCGTTGATAGCACTGCCTTGTTTTTAAGTGG20.1932367149758454No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGACCCATG20.1932367149758454No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCTGT20.1932367149758454No Hit
AAATATCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG20.1932367149758454No Hit
AAATATCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG20.1932367149758454No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACAATG20.1932367149758454No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTCCCATGTACTCTGC20.1932367149758454Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACATGT20.1932367149758454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)