FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460
Total Bases211.6 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC85558.56164383561644No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC29420.136986301369863No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC261.7808219178082192No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT211.4383561643835616No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT181.2328767123287672No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT151.0273972602739725No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTGGACAGACCAAGTCTCT110.7534246575342466No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT90.6164383561643836No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT80.547945205479452No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT80.547945205479452No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTTTGGACAGACCAA70.4794520547945206No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT60.410958904109589No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACAAAGTCTC60.410958904109589No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT60.410958904109589No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGATTTTTGGACAGACCAAGTCTCT50.3424657534246575No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGTACATGG50.3424657534246575Clontech Universal Primer Mix Long (96% over 27bp)
ACTTAAAAACAAGGCAGTTCTAACGGTATTTTTGGACAGACCAAGTCTCT50.3424657534246575No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT40.273972602739726No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT40.273972602739726No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT40.273972602739726No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGAAGAGGTCCATT40.273972602739726Clontech SMARTer II A Oligonucleotide (95% over 22bp)
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGGTCCATT30.2054794520547945Clontech Universal Primer Mix Long (96% over 26bp)
ACTTAAAAACAAGGCAGTTCTGAACGGATTTTTGGACAGACCAAGTCTCT30.2054794520547945No Hit
ACTTAAAAACAAGGCAGTTCTGAACGTATTTTTGGACAGACCAAGTCTCT30.2054794520547945No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTTTGGACAGACCAAGTCTCT30.2054794520547945No Hit
ACTTAAAAAAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT30.2054794520547945No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTTTGGACAGACCAAGTCTCT30.2054794520547945No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACACACCAAGTCTC20.136986301369863No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGCCCAAGTCTC20.136986301369863No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTTTTTTGGACAGACCAAGTCTCT20.136986301369863No Hit
ACTTAAAAACAAGGAAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC20.136986301369863No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTTTGGACAGACCAAGTCTCT20.136986301369863No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGT20.136986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.136986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGCCTGAACGGTATTTTTGGACAGACCAAGTCTCT20.136986301369863No Hit
ACTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT20.136986301369863No Hit
ACTTAAAAACATGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC20.136986301369863No Hit
ACTTAAAAAAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT20.136986301369863No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGTACATGGAAGCAGTGGT20.136986301369863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)