FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460
Total Bases211.6 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG85358.42465753424657No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG28919.794520547945204No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG291.9863013698630139No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT201.36986301369863No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG171.1643835616438356No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT171.1643835616438356No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.7534246575342466No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT80.547945205479452No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT70.4794520547945206No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAGGCTAATGTAGAA70.4794520547945206No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT70.4794520547945206No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.410958904109589No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC60.410958904109589Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.410958904109589No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTCCCATGTACTCTGC60.410958904109589Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AAATCCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.3424657534246575No Hit
AAATACCGTTAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.3424657534246575No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG40.273972602739726No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATGGCTAATGTAGAACCATGT40.273972602739726No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA40.273972602739726Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.273972602739726No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.2054794520547945No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCATGTACTCTGC30.2054794520547945Clontech Universal Primer Mix Long (95% over 23bp)
AAATACGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.2054794520547945No Hit
AAATCCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.2054794520547945No Hit
AAATACCGTTCAGAGCTGCCTTTTTTTAAGTGGCTAATGTAGAACCATGT30.2054794520547945No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTACCCACAGAAACA30.2054794520547945Clontech Universal Primer Mix Long (95% over 23bp)
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.2054794520547945No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAAACATG20.136986301369863No Hit
AAATACCGTTCAGGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.136986301369863No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAAAGTAGAACCATG20.136986301369863No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.136986301369863No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.136986301369863No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGACCCATG20.136986301369863No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT20.136986301369863No Hit
AAATACCGTTCAGAACTGCCTTTTTTTAAGTGGCTAATGTAGAACCATGT20.136986301369863No Hit
AAATACCGTTCAGAGCTGCCATGTTTTTAAGTGGCTAATGTAGAACCATG20.136986301369863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)