FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24771
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA1126545.47656533850067No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA298312.04230753703928No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC256410.350813451213112No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT23539.499010940212345No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1400.5651770215170966No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1360.5490291066166082No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1300.5248072342658754No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1240.5005853619151427No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1100.44406765976343304No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1100.44406765976343304No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1080.43599370231318885No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG850.3431431916353801No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT800.3229582980097695No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT780.31488434055952524No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT740.29873642565903674No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC730.29469944693391464No Hit
TGCCATCTTGCCACATCTGCACCCGGGTGGAGCCGCCGCAGGTGCAGATC590.238181744782205No Hit
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGG410.16551612773000685Clontech Universal Primer Mix Long (96% over 27bp)
TGCCATCTTGCCACATCTACTCCTTCTTCAAGGGTATCCGCCAACTTGAG370.14936821282951837No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA350.14129425537927415Clontech Universal Primer Mix Long (96% over 25bp)
AAGGTGGAGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT350.14129425537927415No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT340.13725727665415205No Hit
AAGGGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT330.13322029792902992No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT330.13322029792902992No Hit
AAGGTGGATGGGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT320.1291833192039078No Hit
TGCCATCTTGCCACATCTTCATAGTTGGGTAGGGTGGCACTGAGACCAAT310.12514634047878567No Hit
TGCCCTCTCTGCCTTTTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG290.11707238302854145No Hit
AAGGTGGATGGTGATGTGGCCTTCAATTTAGCCACCTGCTTCCGGGCTGA290.11707238302854145No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT280.11303540430341932No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT280.11303540430341932No Hit
AAGGTGGATGGTGATGTGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT250.10092446812805297No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCCGCTTCCGGGCTGA250.10092446812805297No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)