FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37496
Total Bases5.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1071528.576381480691275No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1062528.33635587796032No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA504213.446767655216558No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT31408.374226584168978No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC19625.232558139534884No Hit
TGCCATCTTGCCACATCTCTTGCTTCCCGGAAGCAGTGGTATCAACGCAG6361.6961809259654361No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2890.7707488798805207No Hit
TGCCATCTTGCCACATCTTTTGCTTCCCGGAAGCAGTGGTATCAACGCAG2710.722743759334329Clontech Universal Primer Mix Long (95% over 22bp)
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2450.6534030296564967No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1620.4320460849157244No Hit
TGCCATCTTGCCACATCTTGCACTTAGTGACCATCATCATGAGAAACTGG1210.32270108811606574No Hit
TGCCATCTTGCCACATCTCTTGCTTCCCGAAAGCAGTGGTATCAACGCAG1050.2800298698527843No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC1040.2773629187113292No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT630.1680179219116706No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT620.1653509707702155No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT500.13334755707275442No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT490.13068060593129932No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT430.11467889908256881No Hit
TGCCATCTTGCCACATCTTTTGCTTCCCGAAAGCAGTGGTATCAACGCAG400.10667804565820355Clontech Universal Primer Mix Long (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)