FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37496
Total Bases5.4 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC57

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC1038627.69895455515255No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1004626.792191167057823No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC473012.614678899082568No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG465112.403989758907617No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2880.7680819287390654No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2420.6454021762321315No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1880.5013868145935566No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT1540.41071047578408365No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCGGGAAGC1270.33870279496479627Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1150.3066993812673352No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA1030.27469596756987413No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG990.26402816300405374No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA960.2560273095796885No Hit
GTTGGCGGATACCCTTCCATACTTTATAAATGTATACCAACTTCACCATC910.24269255387241306No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT880.2346917004480478No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC770.20535523789204183Clontech Universal Primer Mix Long (96% over 26bp)
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG670.17868572647749092No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA660.17601877533603583No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG660.17601877533603583No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA610.16268401962876042No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA600.16001706848730532No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT580.15468316620439515No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG570.15201621506294005No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT560.14934926392148495No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA510.13601450821420952No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG490.13068060593129932No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA480.12801365478984425No Hit
GTTGGCGGATACCCTTCCGTACTTTATAAATGTATACCAACTTCACCATC460.12267975250693408No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG460.12267975250693408No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC400.10667804565820355No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA380.10134414337529336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)