FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18550
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT844145.504043126684635No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA256613.83288409703504No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC246613.29380053908356No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT13047.0296495956873315No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA2121.1428571428571428No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2051.1051212938005393No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1810.9757412398921833No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1720.9272237196765499No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC1200.646900269541779No Hit
TGCCATCTTGCCACATCTTCCCAAGCAGTGGTATCAACGCAGAGTACATG730.39353099730458224Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTACACATCAAGGGTATCCGCCAACTTGAGGAGA680.3665768194070081No Hit
TGCCATCTTGCCACATCTCTCTGATCGTTTTTTCACTGACCCGGTGAGGC430.23180592991913748No Hit
TGCCATCTTGCCACATCTGTATGAACTATACCTTGGGTAACTAACTGATC410.22102425876010784No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT360.1940700808625337No Hit
TGCCATCTTGCCACATCTCCCCAAGCAGTGGTATCAACGCAGAGTACATG350.18867924528301888Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT350.18867924528301888No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG340.18328840970350405No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT300.16172506738544476No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT300.16172506738544476No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT290.15633423180592992No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.1509433962264151No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT260.14016172506738545No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT250.13477088948787064No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT230.12398921832884097No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT230.12398921832884097No Hit
TGCCATCTTGCCACATCTTACTTCAGTGAAATTAAGTACCAAATTCCAGT220.11859838274932614No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT210.11320754716981132No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT210.11320754716981132No Hit
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGG210.11320754716981132Clontech Universal Primer Mix Long (96% over 27bp)
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG210.11320754716981132No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA200.1078167115902965No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT200.1078167115902965No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC200.1078167115902965No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG190.10242587601078167No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTCGGTTTCCCAGT190.10242587601078167No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT190.10242587601078167No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)