FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28802
Total Bases4.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT965933.535865564891324No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA616621.408235539198667No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTCAGCCACCTGCTTCCGGGCTGA496717.24533018540379No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT27999.718075133671272No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC16975.891951947781404No Hit
TGCCATCTTGCCACATCTTAGACTATTTTTCTTTTCTCTTCCCATACTCT1290.4478855635025345No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1040.36108603569196585No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG870.30206235678077914No Hit
AAGGTGGATGTGATGTGGTCTTCAATTCAGCCACCTGCTTCCGGGCTGAT730.25345462120686063No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT670.23262273453232415No Hit
TGCCATCTTGCCACATCTACAACAGGACCCAGCTGGCCAAGGGTATCCGC650.22567877230747863No Hit
AGGTGGATGGTGATGTGGTCTTCAATTCAGCCACCTGCTTCCGGGCTGAT610.21179084785778765No Hit
AAGGTGATGGTGATGTGGTCTTCAATTCAGCCACCTGCTTCCGGGCTGAT610.21179084785778765No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC530.18401499895840567No Hit
AAGTGGATGGTGATGTGGTCTTCAATTCAGCCACCTGCTTCCGGGCTGAT460.15971113117144645No Hit
TGCCCTCTCTGCCTTTTCTCCCCAGGGTATTTGGTTTCCCAGTCCACTAT400.13887924449690994No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT350.12151933893479619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)