FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9341
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC547158.56974627984156No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC117412.56824751097313No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG5986.401884166577454No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1161.241837062413018No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC930.9956107483138851No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT610.6530350069585698No Hit
GTTGGCGGATACCCTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAA320.34257574135531527No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT270.28904828176854724No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT250.26763729793384006No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT250.26763729793384006No Hit
GTGTCGCTACCTCAGTTTGCACACTGACCACATCACCATCCACCTTAACT220.23552082218177925No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC210.22481533026442566No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC210.22481533026442566No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA190.20340434642971847No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACATGCAAAATAC190.20340434642971847No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATAC160.17128787067765763No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC150.16058237876030404No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATAC140.14987688684295042No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT140.14987688684295042No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTAC140.14987688684295042No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA130.13917139492559683No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT120.12846590300824323No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACGCACGCAAAATAC120.12846590300824323No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA120.12846590300824323No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATGC120.12846590300824323No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACAC120.12846590300824323No Hit
GGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACT110.11776041109088962No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC110.11776041109088962No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG110.11776041109088962No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCGAAATAC110.11776041109088962No Hit
GTTGGCGGATACCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCA110.11776041109088962No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA110.11776041109088962No Hit
GTGTCGCTACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACT110.11776041109088962No Hit
GTGTGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACT100.10705491917353603No Hit
GTTGGCGGATACCCTTCCATAGCCACCGTCCTGCTGTCTATATCAACCAA100.10705491917353603No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)