FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27435
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1012336.89812283579369No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT930533.916529979952614No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT338312.330964096956443No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC12614.596318571168216No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2140.7800255148532896No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1970.718060871149991No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA1460.5321669400400948No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT570.20776380535811917No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT520.18953891015126662No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT410.149444140696191No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC400.14579916165482049No Hit
TGCCATCTTGCCACATCTTACCGCGCAAGGGTATCCGCCAACTTGAGGAG330.1202843083652269No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTTCCAGTCCACTA290.10570439219974485No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT290.10570439219974485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)