FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19687
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT709736.04916950271753No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA446822.695179560115815No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA241712.277137197135165No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC12666.4306395083049726No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT8654.393762381266826No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG3571.8133793874130135No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2751.3968608726570833No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2111.071773251384162No Hit
TGCCATCTTGCCACATCTCCCCCGGGGGCTTCTATTGCATTTGCCTGGAA1260.6400162543810637No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1030.5231878904861076No Hit
TGCCATCTTGCCACATCTTCCCCGGGGGCTTCTATTGCATTTGCCTGGAA720.36572357393203636No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT700.3555645857672576No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG530.2692131863666379No Hit
TGCCATCTTGCCACATCTCCCCCGGGGGCTTCTATTGCATTTGCATGGAA530.2692131863666379No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT520.2641336922842485No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT480.2438157159546909No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC430.21841824554274394No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT390.19810026921318635No Hit
TGCCATCTTGCCACATCTTCCCCGGGGGCTTCTATTGCATTTGCATGGAA330.16762330471885No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.14222583430690303No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT230.11682836389495606No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC230.11682836389495606No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT210.10666937573017728No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG210.10666937573017728No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)