FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34259
Total Bases4.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1251136.51887095361803No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA654619.107387839691757No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA514115.006275723167633No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT31979.331854403222511No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC21486.269885285618377No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT3661.068332409002014No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3531.0303861758953852No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2690.7851951312063984No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC1120.32692139291864913No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT840.24519104468898684No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT690.2014069295659535No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT640.18681222452494234No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT610.1780554015003357No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT420.12259552234449342No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT350.10216293528707784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)