FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27094
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1083439.98671292537093No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA824730.43847346275928No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT333912.323761718461652No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC12104.4659334169926925No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG2630.9706946187347752No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1030.38015796855392336No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG720.26574149258138335No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT490.18085184911788588No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT420.1550158706724736No Hit
TGCCATCTTGCCACATCTTGCGGAAGGGTATCCGCCAACTTGAGGAGACC400.14763416254521297No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC400.14763416254521297No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT360.13287074629069168No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT320.11810733003617038No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC290.1070347678452794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)