FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27094
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1023337.768509633129106No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC804729.700302650033215No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG396514.634236362294233No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG2540.9374769321621023No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1160.4281390713811176No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1050.38753967668118405No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1010.37277626042666273No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG800.29526832509042594No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAGGATTCG770.28419576289953496No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC700.25835978445412267No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG660.24359636819960137No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG650.23990551413597105No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG590.21776038975418913No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA560.20668782756329815No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA560.20668782756329815No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA500.1845427031815162No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT490.18085184911788588No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA480.17716099505425556No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA480.17716099505425556No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT450.16608843286336458No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG430.15870672473610395No Hit
GTTGGCGGATACCCTTCCGCAAGATGTGGCAAGATGGCAAACTGGTGTAG420.1550158706724736No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG420.1550158706724736No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA410.1513250166088433No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA400.14763416254521297No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATAC370.136561600354322No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG370.136561600354322No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA350.12917989222706133No Hit
GTTGGCGGATACCCTTCCATAAGGCTTTAACACAGAATCAAAAGATTCGA330.12179818409980069No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC330.12179818409980069No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG320.11810733003617038No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA320.11810733003617038No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT310.11441647597254005No Hit
GTTGGCGGATACCCTTCCACACACGCAAAATACTCCTTCAGCGGAGCGAA290.1070347678452794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)