FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13036
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA425132.609696225836146No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT340026.08162012887389No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC133910.27155569193004No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG8416.451365449524395No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT3782.8996624731512735No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA3342.5621356244246702No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1190.9128567045105861No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1100.8438171218165081No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG1100.8438171218165081No Hit
TGCCATCTTGCCACATCTACACATCAAGGGTATCCGCCAACTTGAGGAGA600.46026388462718626No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT400.3068425897514575No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA360.27615833077631174No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG300.23013194231359313No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG270.2071187480822338No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACTTGAGGA240.18410555385087451No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC200.15342129487572875No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT200.15342129487572875No Hit
TGCCATCTTGCCACATCTTGAGATGCTGGCTGGCTGATATCAGAACGTGG170.13040810064436945No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATTTGGTTTCCCAGTC160.122737035900583No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTACCGCCAACTTGAGGA160.122737035900583No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACTTGAGGA160.122737035900583No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)