FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10959
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG337130.76010584907382No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC242822.155306141071264No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC225620.5858198740761No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1451.323113422757551No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1271.1588648599324756No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA780.7117437722419928No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA700.6387444109864039No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT640.5839948900447121No Hit
GTGTCGCTACCTCAGTTTGCACACTGACCACATCACCATCCACCTTAACT570.5201204489460717No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT570.5201204489460717No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA430.3923715667487909No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT410.3741217264348937No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA390.3558718861209964No Hit
GTTGGCGGATACCCTTCCATGTAGGGCAGGATTTGTTGTGTAGTGAGGTC370.3376220458070992No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA330.3011223651793047No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA320.29199744502235603No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA300.27374760470845877No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCTTCCTGTGTTGGCGG250.22812300392371565No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT230.2098731636098184No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA230.2098731636098184No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA220.2007482434528698No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT220.2007482434528698No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT210.19162332329592116No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA200.18249840313897253No Hit
GTTGGCGGATACCCTTCAATGTAGGGCAGGATTTGTTGTGTAGTGAGGTC190.1733734829820239No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG190.1733734829820239No Hit
GTTGGCGGATACCCTTCCATCTTGTCTGAGCCCTAAATGCCTACTTACTT190.1733734829820239No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT180.16424856282507527No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC180.16424856282507527No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA150.13687380235422938No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT140.12774888219728078No Hit
TACCTCAGTTTGCCCCCATGTACCTTGGAACTAGTTGATCTCAGAGGTCC140.12774888219728078No Hit
GTTGGCGGATACTCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG130.11862396204033215No Hit
CAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT110.1003741217264349No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAGGATTCG110.1003741217264349No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)