FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3075
Total Bases445.4 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC144947.12195121951219No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC42813.918699186991871No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1454.715447154471545No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1223.967479674796748No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG993.2195121951219514No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT341.1056910569105691No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT250.8130081300813009No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT160.5203252032520325No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT130.42276422764227645No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG130.42276422764227645No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT110.35772357723577236No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC110.35772357723577236No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC110.35772357723577236No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT100.3252032520325203No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT100.3252032520325203No Hit
TACCTCAGTTTGCCCCCATGGATAAGGGAGGATTACTGTAACACAAGATG90.2926829268292683No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA90.2926829268292683No Hit
GAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT80.26016260162601623No Hit
TACCTCAGTTTGCCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGT80.26016260162601623No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA70.22764227642276422No Hit
GTTGGCGGATACCCTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAA70.22764227642276422No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT70.22764227642276422No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAACAC60.1951219512195122No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCCC60.1951219512195122No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG60.1951219512195122No Hit
CAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT50.16260162601626016No Hit
GTTGGCGGATACCCTTCCATATGGGATGAAGTTGGGGAGAGAGATGTGGC50.16260162601626016No Hit
CGAAGTAGACTAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC50.16260162601626016No Hit
CGAAGTAGACCAGCGGCACCTCGGCCCCACCCTGGACCTTAGCTCAGCAC50.16260162601626016No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACACTGGACCTTAGCTCAGCAC50.16260162601626016No Hit
CGAAGTAGACCACGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT50.16260162601626016No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT50.16260162601626016No Hit
GTTGGCGGATACCCTTCCAACTCTAAAATGTTATAATTCTAAGATGTGGC50.16260162601626016No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA50.16260162601626016No Hit
GTTGGCGGATACCCTTCCATCAATTCCTTTAAGTTTCAGCTTTGCAACCA50.16260162601626016No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG50.16260162601626016No Hit
GTTGGCGGATACCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCA50.16260162601626016No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCAGGACCTTAGCTCAGCAC40.13008130081300812No Hit
TACCTCAGTTTGCCCCAGTGCACCATAAGATGTCCTCAACTGGTGTAGAA40.13008130081300812No Hit
GTTGGCGGATACCCTTCCATGCCCCACGTGTCCTCATTCTTCTTCAGTGC40.13008130081300812No Hit
GTTGGCGGATACCCTTCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTT40.13008130081300812No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGAAC40.13008130081300812No Hit
TACCTCAGTTTGCCCCCGTGCACCATAAGATGTCCTCAACTGGTGTAGAA40.13008130081300812No Hit
CGAAGTAGACCAGCGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT40.13008130081300812No Hit
CGAAGAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT40.13008130081300812No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)