FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12909
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC475636.84251297544348No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA414432.10163451855295No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT4873.772561778604075No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG3852.9824153691223176No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1891.464094817569138No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1721.3324037493221783No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG1341.0380354791230924No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA970.7514137423502982Clontech Universal Primer Mix Long (96% over 25bp)
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT570.4415524052986289No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT560.4338058718723371No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG530.41056627159346193No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT420.32535440390425285No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT410.31760787047796113No Hit
TGCCATCTTGCCACATCTTAGACAAATCAACTACAAAAATCATTAAAAAG400.3098613370516694No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC290.2246494693624603No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT270.20915640250987683No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT260.2014098690835851No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT240.18591680223100163No Hit
TGCCATCTTGCCACATCTTCTCCATTCATGGGACTAGCCATCGCAGATGC240.18591680223100163No Hit
TGCCATCTTGCCACATCTTCTTTTTCCCCAGCACTGATCCAAAATATAGA240.18591680223100163No Hit
TGCCATCTTGCCACATCTTCTTCTTCATCAAGGGTATCCGCCAACTTGAG220.17042373537841815No Hit
TGCCTCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT200.1549306685258347No Hit
TGCCATCTTGCCACATCTGCGAAACCACAGCCAAGGGTATCCGCCAACTT200.1549306685258347No Hit
TGCCATCTTGCCACATCTGTTCTGAACTGTTAAAATCATCTTCTGAGTCC200.1549306685258347No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA180.13943760167325123No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG170.13169106824695948No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT170.13169106824695948No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT170.13169106824695948No Hit
TGCCACTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT160.12394453482066775No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGCC150.11619800139437601No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACGTGGTC150.11619800139437601No Hit
TGCCATCTTGCCAATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT150.11619800139437601No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC150.11619800139437601No Hit
TGCCATCTTGCCACATCTTGGTATCAACGCAGAGTACATGGGTATCCGCC140.10845146796808428No Hit
TGCCATCTTGCCACATCTTCCTCCCAAAGCAGTGGTATCAACGCAGAGTA140.10845146796808428Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC130.10070493454179255No Hit
TGCCATCTTGCCACATCTCTTCAAACTTGGCTGCTCGTGACCGCCATTCT130.10070493454179255No Hit
TGCCATCTTGCCACATCTTAGGAGTTTAGTTGAAATCATTGAACATGGTC130.10070493454179255No Hit
GCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT130.10070493454179255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)