FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12909
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG474336.741808040901695No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC407631.574870245565112No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG3822.959175768843443No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1911.4795878844217214No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1711.3246572158958867No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1601.2394453482066776No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1230.9528236114338834No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1160.8985978774498412No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA860.6662018746610892No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA720.5577504066930049No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA600.46479200557750405No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA580.4492989387249206No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT570.4415524052986289No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCAACTG510.3950732047408785No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA480.37183360446200325No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA410.31760787047796113No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA390.3021148036253776No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA280.21690293593616855No Hit
TACCTCAGTTTGCCCCCATGCCCCCATGCCCCTCTGAGTCACACAGGACA270.20915640250987683No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA260.2014098690835851No Hit
TACCTCAGTTTGCCCCCATGTGTAGGGGACACAGAACCCTTTGAGAAAAC240.18591680223100163No Hit
GTTGGCGGATACCCTTGATGAAGAAGAAGATGTGGCAAGATGGCAAACTG230.1781702688047099No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC210.16267720195212643No Hit
GTTGGCGGATACCCTTGGCTGTGGTTTCGCAGATGTGGCAAGATGGCAAA200.1549306685258347No Hit
GTTGGCGGATACCCTTCCGACGCATCTGCGATGGCTAGTCCCATGAATGG190.14718413509954295No Hit
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT170.13169106824695948No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC170.13169106824695948No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCAAA170.13169106824695948No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG150.11619800139437601No Hit
GTTGGCGGATACCCTTCCATAAAGGCCTTAACACAGAATCAAAAGATTCG150.11619800139437601No Hit
GTTGGCGGATACCCTTCTCCTTCTATATTTTGGATCAGTGCTGGGGAAAA140.10845146796808428No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCCATAAAGGCTTTA140.10845146796808428No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTGGGGAGGAA140.10845146796808428Clontech SMART CDS Primer II A (96% over 26bp)
TACCTCAGTTTGCCCCCATGCCCCTCTGAGTCACACAGGACAGAGGAGGC140.10845146796808428No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC130.10070493454179255Clontech Universal Primer Mix Long (96% over 26bp)
TACCTCAGTTTGCCCCCATGCAAACTGATGAACAGACAAACAAGGAGCAG130.10070493454179255No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT130.10070493454179255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)