FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26749
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1152643.08946128827246No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA564221.092377285132155No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA335012.523832666641743No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC14685.4880556282477855No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT12614.714194923174698No Hit
TGCCATCTTGCCACATCTCCAGGGTTCCGACCCGATCGAGCACTCCGAGG2060.7701222475606564No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1810.6766608097498972No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1390.5196455942278216No Hit
TGCCATCTTGCCACATCTTCAGGGTTCCGACCCGATCGAGCACTCCGAGG1100.4112303263673408No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT490.1831844181090882No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT450.1682305880593667No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGCACGAGT430.16075367303450597No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG410.15327675800964521No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT360.13458447044749336No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC360.13458447044749336No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT320.11963064039777188No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT300.11215372537291114No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT300.11215372537291114No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.10467681034805039No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT280.10467681034805039No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT280.10467681034805039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)