FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26749
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length53-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1095740.962278963699575No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC546220.419454932894688No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC313011.701372013907061No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG25039.357359153613219No Hit
GTTGGCGGATACCCTTCCATCATGCCGTCGAGGCCTACAAAATCTCGGAC2741.0243373584059219No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1760.6579685221877453No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1360.5084302216905305No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG910.3401996336311638No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG760.2841227709447082No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT740.27664585591984747No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG620.231784365770683No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA610.22804590825825266No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA610.22804590825825266No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG600.22430745074582228No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG520.1943997906463793No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG410.15327675800964521No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA390.1457998429847845No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA390.1457998429847845No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG380.1420613854723541No Hit
GTTGGCGGATACCCTTCCATTCACCAAAGATGATTGCTTTAATAGTTGTT370.13832292795992374No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG370.13832292795992374No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA360.13458447044749336No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT340.12710755542263263No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG330.12336909791020226No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA320.11963064039777188No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA300.11215372537291114No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT270.10093835283562001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)