FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21820
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT706332.36938588450962No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA593027.176901924839598No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA432719.830430797433547No Hit
TGCCATCTTGCCACATCTTAGTGGTATCAACGCAGAGTACATGGGAAGCA4512.066911090742438Clontech SMARTer II A Oligonucleotide (100% over 21bp)
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC4331.984417965169569No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT1740.7974335472043996No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1700.7791017415215399No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1570.7195233730522457No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT680.31164069660861593No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT620.2841429880843263No Hit
TGCCATCTTGCCACATCTTAGTGGTATCAACGCAGAGTACATGGGTATCC590.27039413382218147Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT590.27039413382218147No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT510.23373052245646195No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT460.21081576535288726No Hit
TGCCATCTTGCCACATCTCAGTGGTATCAACGCAGAGTACATGGGAAGCA430.19706691109074242Clontech SMARTer II A Oligonucleotide (95% over 23bp)
TGCCATCTTGCCACATCGCAGTGGTATCAACGCAGAGTACATGGGAAGCA410.18790100824931255Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC370.1695692025664528No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT360.16498625114573787No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT340.15582034830430797No Hit
TGCCATCTTGCCACATCTGTCTACGGCCATACCACCCTGAACGCGCCCGA320.1466544454628781No Hit
TGCCATCTTGCCACATCTTAGCTTAAGGGTATCCGCCAACTTGAGGAGAC280.12832263978001834No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT240.10999083409715857No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT230.10540788267644363No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT220.10082493125572868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)