FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9422
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC610864.82700063680747No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC111611.84461897686266No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG2362.5047760560390575No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1081.146253449373806No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1081.146253449373806No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT830.880917002759499No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA560.5943536404160475No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT340.36085756739545743No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT280.2971768202080238No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT230.24410953088516238No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATAC200.21226915729144555No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC190.2016556994268733No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC180.19104224156230099No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC170.18042878369772872No Hit
CGAAGTAGACCAGCGGCATCACCATCCACCTTAACTGGTGTAGAACCATG150.15920186796858415No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACATACGCAAAATAC140.1485884101040119No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTAC140.1485884101040119No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC140.1485884101040119No Hit
GTTGGCGGATACCCTTGATGGCACAAGATGTGGCAAGATGGCAAACTGGT130.13797495223943962No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTCACACACACGCAAAATAC130.13797495223943962No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT130.13797495223943962No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG120.12736149437486732No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT120.12736149437486732No Hit
GTGTCGCTACCTCAGTTTGCCTCCATGTCCCTTACACACACGCAAAATAC120.12736149437486732No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT110.11674803651029506No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC110.11674803651029506Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA110.11674803651029506No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT110.11674803651029506No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT110.11674803651029506No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATAC100.10613457864572277No Hit
GTGTCGCTACCTCAGTTTGCACCCATGTCCCTTACACACACGCAAAATAC100.10613457864572277No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT100.10613457864572277No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)