FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12437
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT427534.373241135322026No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA262121.074214038755326No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG4783.843370587762322No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3753.0151965908177214No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT2191.7608748090375492No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA2071.6643885181313822No Hit
TGCCATCTTGCCACATCTAACTATCACCAAAGCTTTCTGTTCCTATCAGA1371.101551821178741No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC1371.101551821178741No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1140.9166197636085872No Hit
TGCCATCTTGCCACATCTTGTTTGAGAGAAGTGGGACCAATGCTGATGAA1070.8603360939133232No Hit
TGCCATCTTGCCACATCTTACTATCACCAAAGCTTTCTGTTCCTATCAGA930.7477687545227949No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC640.5145935514995578No Hit
TGCCATCTTGCCACATCTTGTATCCAATCTCAATGTTAAGCTATAGTGAA560.450269357562113No Hit
TGCCATCTTGCCACATCTCTCAAAGGAATGGAAGGGTATCCGCCAACTTG490.3939856878668489No Hit
TGCCATCTTGCCACATCTGCTTTGGTAAAAGAAGCTGCCCAATTTTTTGG480.3859451636246683No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG420.3377020181715848No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC330.2653372999919595No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAGGCAAGGGTATCCGCCA300.24121572726541768No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT270.21709415453887593No Hit
TGCCATCTTGCCACATCTTGGCCGGTTGGCTACGTTCGGAAGGGTATCCG270.21709415453887593No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAGGAAGGGTATCCGCCAA260.20905363029669535No Hit
TGCCATCTTGCCACATCTTGAGAGGCGTAGGTGATGGGAAGCAGTGGTAT220.17689153332797297No Hit
TGCCATCTTGCCACATCTTAGCCGGTTGGCTACGTTCGGAAGGGTATCCG220.17689153332797297No Hit
TGCCATCTTGCCACATCTTTTCCCACTTAGCACAGGCTTCGGGAAGGGTA220.17689153332797297No Hit
TGCCATCTTGCCACATCTGTATCCAATCTCAATGTTAAGCTATAGTGAAG190.15276996060143122No Hit
TGCCATCTTGCCACATCTTAGACTAGTTTGTTAAGGGTATCCGCCAACTT190.15276996060143122No Hit
TGCCATCTTGCCACATCTTGGAAGGAGTGGTGGAAGGGTATCCGCCAACT190.15276996060143122No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAGGGAAGGGTATCCGCCA190.15276996060143122No Hit
TGCCATCTTGCCACATCTTGCACCCCAATGCGGACCAGCCGCCGCAGCAG180.14472943635925062No Hit
TGCCATCTTGCCACATCTCGAAAGTCCAAGGGTATCCGCCAACTTGAGGA170.13668891211707002No Hit
GAGGACATCTTATGGTGCACTGCTGGGTAGCTACGTATGGAAGGGTATCC170.13668891211707002No Hit
TGCCATCTTGCCACATCTCGGCCGGTTGGCTACGTTCGGAAGGGTATCCG160.12864838787488944No Hit
TGCCATCTTGCCACATCTGACACTGTCTCCCAAGGGTATCCGCCAACTTG160.12864838787488944No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT150.12060786363270884No Hit
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGG150.12060786363270884Clontech Universal Primer Mix Long (96% over 27bp)
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG150.12060786363270884Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGTATCCGCCAA150.12060786363270884No Hit
TGCCATCTTGCCACATCTAGCACCCCAATGCGGACCAGCCGCCGCAGCAG150.12060786363270884No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAA140.11256733939052825Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTGACAGGGGGAGGATGTTGGAGCCTGAAAGCC140.11256733939052825No Hit
TGCCATCTTGCCACATCTTAGAAGGAGTGGTGGAAGGGTATCCGCCAACT140.11256733939052825No Hit
TGCCATCTTGCCACATCTTTCAAAGGAATGGAAGGGTATCCGCCAACTTG140.11256733939052825No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAAGCAGTGGTATCAACGC140.11256733939052825Clontech Universal Primer Mix Long (95% over 21bp)
TGCCATCTTGCCACATCTTAGGAACCCCCATGGGTGCCGTTAATCCCCTC140.11256733939052825No Hit
TGCCATCTTGCCACATCTCGAGAGGCGTAGGTGATGGGAAGCAGTGGTAT140.11256733939052825No Hit
GAGGACATCTTATGGTGCACTGCTGGGTAGCTACGTTTGGAAGGGTATCC130.10452681514834768No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC130.10452681514834768No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)