FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12437
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC57

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA409632.9339872959717No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC255320.527458390287045No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC4683.762965345340516No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC3702.9749939696068184No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG3122.5086435635603443No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC2021.624185896920479No Hit
GTTGGCGGATACCCTTCCCTGTGTCACCCCTCTGATAGGAACAGAAAGCT1531.2302002090536304No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT1110.8924981908820455No Hit
GTTGGCGGATACCCTTCCAATGAATATAGAACACACCATGGGAAAGAGTT980.7879713757336978No Hit
TACCTCAGTTTGCCCCCATGAACCTTCACTATAGCTTAACATTGAGATTG840.6754040363431696No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA650.5226340757417384No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT620.4985125030151966No Hit
GTTGGCGGATACCCTTCCATTCCTTTGAGAGATGTGGCAAGATGGCAAAC490.3939856878668489No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC420.3377020181715848Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTCCAAAAAATTGGGCAGCTTCTTTTACCAAAGCAG420.3377020181715848No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG410.3296614939294042No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC410.3296614939294042No Hit
GTTGGCGGATACCCTTCTGCCTTTACACTCTGCGAATGATGTCCAACCCT360.28945887271850124No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG350.28141834847632063No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG330.2653372999919595No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG300.24121572726541768No Hit
GTTGGCGGATACCCTTCCATGTGTCACCCCTCTGATAGGAACAGAAAGCT270.21709415453887593No Hit
GTTGGCGGATACCCTTCGAGAAATCTCGTCTCGACCAAGAGCTGAAGCTG260.20905363029669535No Hit
GTTGGCGGATACCCTTCCGAACGTAGCCAACCGGCCAAGATGTGGCAAGA250.20101310605451475No Hit
GTTGGCGGATACCCTTCCTCCCTCGCGCCAGGTAGATGTGGCAAGATGGC240.19297258181233415No Hit
GTTGGCGGATACCCTTGCCTCCCTCGCGCCAGGTAGATGTGGCAAGATGG230.18493205757015357No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG220.17689153332797297No Hit
GTTGGCGGATACCCTTCCGAACGTAGCCAACCGGCTAAGATGTGGCAAGA200.1608104848436118No Hit
GTTGGCGGATACCCTTAACAAACTAGTCTAAGATGTGGCAAGATGGCAAA200.1608104848436118No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG200.1608104848436118No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC200.1608104848436118Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTCCACCACTCCTTCCAAGATGTGGCAAGATGGCAA190.15276996060143122No Hit
GTTGGCGGATACCCTTCCCTCCCTCGCGCCAGGTAGATGTGGCAAGATGG180.14472943635925062No Hit
GTTGGCGGATACCCTTCCCGAAGCCTGTGCTAAGTGGGAAAAGATGTGGC180.14472943635925062No Hit
GTTGGCGGATACCCTTGGACTTTCGAGATGTGGCAAGATGGCAAACTGGT170.13668891211707002No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT160.12864838787488944No Hit
GTTGGCGGATACCCTTCCGAACGTAGCCAACCGGCCGAGATGTGGCAAGA160.12864838787488944No Hit
GTTGGCGGATACCCTTGGGAGACAGTGTCAGATGTGGCAAGATGGCAAAC160.12864838787488944No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA150.12060786363270884No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT150.12060786363270884No Hit
GTTGGCGGATACCCTTCCACCACTCCTTCTAAGATGTGGCAAGATGGCAA140.11256733939052825No Hit
GTTGGCGGATACCCTTCCATTCCTTTGAAAGATGTGGCAAGATGGCAAAC140.11256733939052825No Hit
GTTGGCGGATACCCTTCCATACGTAGCTACCCAGCAGTGCACCATAAGAT140.11256733939052825No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG140.11256733939052825No Hit
TACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATACTCCTTCA140.11256733939052825No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCATGTACTCTGCGTTG140.11256733939052825No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA140.11256733939052825No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG130.10452681514834768No Hit
TACCTCAGTTTGCCCCCATGGGCTCCTCTGTGGTTCACTAATTGTGACCT130.10452681514834768No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA130.10452681514834768No Hit
GTTGGCGGATACCCTTGGGAGAGGAGGGGATTAACGGCACCCATGGGGGT130.10452681514834768No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)