FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8899
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC196122.036183840880998No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA170019.10327003034049No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA152817.170468591976626No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT108712.214855601753007No Hit
TGCCATCTTGCCACATCTTAGGGACCTACAGCTAAGGCCCCTAATTCGTG5225.865827621081021No Hit
TGCCATCTTGCCACATCTTAGAGACCTACAGCTAAGGCCCCTAATTCGTG1942.1800202269917968No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1711.921564220698955No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1301.460838296437802No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT961.0787728958309923No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG810.9102146308573996No Hit
TGCCATCTTGCCACATCTGACCCCATGATATGTATTGTTAACTGACAGAA430.4832003595909653No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT370.4157770536015282No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC300.33711652994718505No Hit
TGCCATCTTGCCACATCTTACCCCATGATATGTATTGTTAACTGACAGAA240.2696932239577481No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT230.25845600629284193No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT230.25845600629284193No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT200.22474435329812337No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT190.21350713563321722No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT190.21350713563321722No Hit
GAGGACATCTTGCCACATCTTAGAGACCTACAGCTAAGGCCCCTAATTCG180.20226991796831106No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG170.19103270030340488No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT150.16855826497359253No Hit
TGCCATCTTGCCACATCTTCAATACTGGGATGAGGTGGTGTTTTCATCTG140.15732104730868637No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT140.15732104730868637No Hit
GAGGACATCTTGCCACATCTTAGGGACCTACAGCTAAGGCCCCTAATTCG140.15732104730868637No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT130.1460838296437802No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT120.13484661197887404No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC120.13484661197887404No Hit
TGCCTCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT110.12360939431396785No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG110.12360939431396785No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT100.11237217664906168No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT100.11237217664906168No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT90.10113495898415553No Hit
AAGGTGGAGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT90.10113495898415553No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)