FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4357
Total Bases631.6 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG173139.7291714482442No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC120427.633692907964196No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1112.547624512279091No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC932.1344962129905896No Hit
GTTGGCGGATACCCTTCCATTCACCAAAGATGATTGCTTTAATAGTTGTT922.111544640807895No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA541.2393848978655038No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA471.078723892586642No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA471.078723892586642No Hit
GTTGGCGGATACCCTTCCATTTCAAAGAACAGAATGCTAAGGAAGATGTG441.0098691760385585No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT290.6655955932981409No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA250.5737893045673629No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA250.5737893045673629No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA220.5049345880192793No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA180.4131282992885013No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG170.39017672710580675No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA130.2983704383750287No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT130.2983704383750287No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA120.2754188661923342No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG120.2754188661923342No Hit
GTTGGCGGATACCCTTCCTTGGCTTCCTGCTCAAGCTGCTCCTCCAGCTG100.22951572182694516No Hit
GTTGGCGGATACCCTTCCACTGGAATTTGGTACTTAATTTCACTGAAGTA100.22951572182694516No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCCATAAAGGCTTTA100.22951572182694516No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAGAGATTCG90.20656414964425066No Hit
GTTGGCGGATACCCTTCCATGGCTTCCTGCTCAAGCTGCTCCTCCAGCTG90.20656414964425066No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT80.18361257746155613No Hit
GTTGGCGGATACCCTTCCATAAAGGCCTTAACACAGAATCAAAAGATTCG80.18361257746155613No Hit
TGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACT80.18361257746155613No Hit
GTTGGCGGATACCCTTGGCAACTAGTGCATTTGGACTGGAACTACTTGGG70.1606610052788616No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC70.1606610052788616No Hit
GTTGGCGGATACTCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG70.1606610052788616No Hit
GTTGGCGATACCCTTCCATTCACCAAAGATGATTGCTTTAATAGTTGTTA60.1377094330961671No Hit
GTTGGCGGATACCCTTGAGGTCAGGAGGTCGAGACCAGCCTGGCCAACAT60.1377094330961671No Hit
TACCTCAGTTTGCCCCCATGATTCAATTACTTCCTCCTGGGTCCTTCCCA60.1377094330961671No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAGGATTCG60.1377094330961671No Hit
GTTGGCGGATACCCTTCTTAAGATCATGAATTACAATATATTCAAATTTG60.1377094330961671No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG60.1377094330961671No Hit
GTTGGCGGATACCCTTGCCACTGGAATTTGGTACTTAATTTCACTGAAGT60.1377094330961671No Hit
GTTGGTGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG50.11475786091347258No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT50.11475786091347258No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)