FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23641
Total Bases3.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA926339.18192969840531No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT547023.137769129901443No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT276311.687322871282941No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA17097.228966625777251No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC14005.921915316610972No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2491.0532549384543801No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1750.7402394145763715No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG480.20303709656951907No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT430.18188739901019416No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC340.14381794340340934No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT320.13535806437967937No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT300.1268981853559494No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA270.11420836682035447No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT260.10997842730848949No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT250.1057484877966245No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA240.10151854828475954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)