FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21793
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA589327.040792915156242No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA588627.008672509521407No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC444120.378103060615793No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT21459.842610012389299No Hit
TGCCATCTTGCCACATCTTAGAAGTGAGTAAGGCTTTTGCACCTTTTAAT2130.977378057174322No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG1280.5873445601798742No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG960.4405084201349057No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT850.3900334969944478No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG850.3900334969944478No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT750.3441472032303951No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT610.2799063919607213No Hit
TGCCATCTTGCCACATCTAGCGGCGCTGTCTGTCTCTGGATTAAACTAAG560.256963245078695No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT510.23402009819666866No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT490.2248428394438581No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT490.2248428394438581No Hit
TGCCATCTTGCCACATCTGTTGTGCTCATTACTGTCCCACAAAGGTCAGA440.2018996925618318No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT370.1697792869269949No Hit
TGCCATCTTGCCACATCTTGCGGCGCTGTCTGTCTCTGGATTAAACTAAG360.16519065755058965No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT350.16060202817418437No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT340.1560133987977791No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT290.13307025191575278No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC280.1284816225393475No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT250.11471573441013169No Hit
AAGGGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT250.11471573441013169No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT250.11471573441013169No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT250.11471573441013169No Hit
TGCCACTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT240.11012710503372643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)