FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12109
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC924676.35642910232059No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC9607.927987447353209No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT460.3798827318523412No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT450.3716244115946816No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACATGCAAAATAC410.3385911305640433No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC400.3303328103063837No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC390.32207449004872407No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA340.2807828887604261No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT320.26426624824510697No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC300.24774960772978777No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC260.21471632669914942No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT250.20645800644148982No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC250.20645800644148982No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATAC240.19819968618383024No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC240.19819968618383024No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT240.19819968618383024No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATAC230.1899413659261706No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACAC220.18168304566851104No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAT210.17342472541085144No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACGCACGCAAAATAC190.15690808489553226No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACGCACACGCAAAATAC170.14039144438021306No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTAC150.12387480386489388No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACGCGCAAAATAC150.12387480386489388No Hit
GTGTCGCTACCTCAGTTTACCCCCATGTCCCTTACACACACGCAAAATAC150.12387480386489388No Hit
GTGTCGCTACCTCAGTTTGCCTCCATGTCCCTTACACACACGCAAAATAC150.12387480386489388No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATGC140.1156164836072343No Hit
GTGTCGCTACCTCAGTTTGCCCCCGTGTCCCTTACACACACGCAAAATAC130.10735816334957471No Hit
GTGTGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACT130.10735816334957471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)