FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18466
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA562630.466803855734863No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA477425.85291887793783No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC333318.049388064551067No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT14808.014729773638038No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1380.7473193978121954No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1300.7039965341709087No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG1150.6227661648434962No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG940.5090436477851186No Hit
TGCCATCTTGCCACATCTTCTCCAGTCTACAAGTTACATGTTCCCACCCA780.42239792050254527No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT660.3574136250406152No Hit
TGCCCTCTCTGCCTTTTCTTCCCCAGGGTATTTGGTTTCCCAGTCCACTA590.3195061193544893No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGCAAGGGT560.3032600454890068No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT560.3032600454890068No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT540.29242932957868517No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT420.22744503411675512No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT420.22744503411675512No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT340.18412217047546844No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC320.17329145456514675No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT310.16787609660998593No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT270.14621466478934259No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT260.14079930683418174No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT230.12455323296869923No Hit
TGCCATCTTGCCACATCTGAACATGGTTTCAAGTCCTTCATGGATTTGCT220.1191378750135384No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGGAAGGGT190.10289180114805589No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT190.10289180114805589No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT190.10289180114805589No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA190.10289180114805589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)