FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24486
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA877735.844972637425464No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT448018.296169239565465No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT338013.803806256636445No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC17837.281711998693131No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG12535.117209834190966No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG3711.5151515151515151No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3171.2946173323531813No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2481.0128236543330882No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA2070.8453810340602793No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1960.8004574042309892No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG1860.7596177407498161No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC960.39206076941926No Hit
TGCCATCTTGCCACATCTACGCAGGTTGTGTTTAGTCCCCAGACAATGCT690.28179367802009314No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAGGAAGGGTATCCGCCAA650.2654578126276239No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA460.1878624520133954No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACTTGAGGA410.16744262027280896No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT360.1470227885322225No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT340.1388548558359879No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATTTGGTTTCCCAGTC330.13477088948787064No Hit
AAGGTGGATGGTGATGTGGTCTCTCTTTGTAGCCTCCTGGGATCAGTAGA320.13068692313975333No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG310.12660295679163602No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT280.11435105774728416No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTACCGCCAACTTGAGGA280.11435105774728416No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAATTGAGGA270.11026709139916686No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACTTGAG270.11026709139916686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)