FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24486
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC857235.00775953606142No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG467119.07620681205587No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA431617.62639875847423No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG12285.015110675488034No Hit
GTTGGCGGATACCCTTCCATTCACCAAAGATGATTGCTTTAATAGTTGTT3091.261945601568243No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC3071.2537776688720086No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2410.9842358898962672No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC1940.7922894715347545No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT1910.7800375724904026No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCAACTG1840.7514498080535816No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1370.5595033896920689No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1310.5349995916033652No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA920.3757249040267908No Hit
TACCTCAGTTTGCCCCCATGGTCCAGTCACCTCCCTTCCTTGACACATGG770.3144654088050315No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA720.294045577064445No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT670.2736257453238585No Hit
GTTGGCGGATACCCTTCCTCCCTCGCGCCAGGTAGATGTGGCAAGATGGC600.24503798088703752No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA560.22870211549456831No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA550.22461814914645103No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA530.21645021645021645No Hit
GTTGGCGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT410.16744262027280896No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA380.15519072122845706No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG370.15110675488033978No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA350.1429388221841052No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT350.1429388221841052No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATAC330.13477088948787064No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG320.13068692313975333No Hit
GTTGGCGGATACCCTTCCACTAGCTGGTATACTTTTTCTACTACACTCAG300.12251899044351876No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT290.11843502409540146No Hit
GTTGGCGGTACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT290.11843502409540146No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA280.11435105774728416No Hit
TTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT280.11435105774728416No Hit
GTTGGCGGATACCCTTTCCATTTCAGTGCACCATAAGATGTCCTCAACTG270.11026709139916686No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG250.10209915870293229No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)