FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9276
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT643169.32945235015092No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC7297.858990944372575No Hit
TGCCATCTTGCCACATCTTAGAATGCAACATCCTGTCCAAGCAGTGGTAT750.8085381630012937No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT660.7115135834411385No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG550.5929279862009487No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT540.5821474773609314No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT520.5605864596808969No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG490.5282449331608452No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC430.4635618801207417No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG370.39887882708063815No Hit
TGCCATCTTGCCACATCTTCTAGCTGCTAATCACTAGACCAGGTCCAAGC300.3234152652005175No Hit
AAGGTGGATGGTGATGTGGTCGAGAAGAGTATGGAAGGGTATCCGCCAAC290.31263475636050025No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT290.31263475636050025No Hit
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGG290.31263475636050025Clontech Universal Primer Mix Long (96% over 27bp)
TTCTGCGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT280.301854247520483No Hit
TGCCATCTTGCCACATCTATGCTCACCCTTACGGAAGGGTATCCGCCAAC270.2910737386804657No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGGTAT270.2910737386804657No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA270.2910737386804657No Hit
AAGGTGGATGGTGATGTGGTCGAGAAGAGTTTGGAAGGGTATCCGCCAAC260.28029322984044847No Hit
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT210.22639068564036222No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGGTGATAT200.21561017680034494No Hit
TTCTCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT190.2048296679603277No Hit
TTCGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT180.19404915912031048No Hit
TGCCATCTTGCCACATCTTAGATGCCTGCTTGCCGCGAGCTGGGTTGGGG180.19404915912031048No Hit
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG180.19404915912031048Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCTGCAGTTTGGCTGATAGTTGATATATTGAGAGAATCTGGATGATATT170.18326865028029324No Hit
TTCTGCAGTTTGGTGAATAGTTGATATATTGAGAGAATCTGGATGATATT160.172488141440276No Hit
TGCCATCTTGCCACATCTATGCTCACCCTTATGGAAGGGTATCCGCCAAC160.172488141440276No Hit
TTCTGCATTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT140.1509271237602415No Hit
TTCTGCAGTTTGGCTAATAGTTGATATATTGAGAGAATCTGGATGATATT130.14014661492022423No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA130.14014661492022423No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAGTCTGGATGATAT120.129366106080207No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGGATCTGGATGATAT120.129366106080207No Hit
TGCCATCTTGCCACATCTTTGCTCACCCTTATGGAAGGGTATCCGCCAAC110.11858559724018973No Hit
TTCTGCAGTTTGGTTGAATAGTTGATATATTGAGAGAATCTGGATGATAT110.11858559724018973No Hit
TTCTGCAGTTTGGCTGAATAGTTGGTATATTGAGAGAATCTGGATGATAT100.10780508840017247No Hit
TGCCATCTTGCCACATCTTCGTCCTTCGTCCAAGGGTATCCGCCAACTTG100.10780508840017247No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGGGAATCTGGATGATAT100.10780508840017247No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)