FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9276
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG638168.79042690815005No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1671.8003449762828805No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1561.6817593790426906No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA971.0457093574816732No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT880.9486847779215178No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA800.8624407072013798No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA720.7761966364812419No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA680.733074601121173No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA590.6360500215610178No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA580.6252695127210005No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC560.603708495040966No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT520.5605864596808969No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC480.517464424320828No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA440.4743423889607589No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA420.45278137128072443No Hit
GTTGGCGGATACCCTTCCATACTCTTCTCGACCACATCACCATCCACCTT270.2910737386804657No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC270.2910737386804657No Hit
GTTGGCGGATACCCTTCCGTAAGGGTGAGCATAGATGTGGCAAGATGGCA240.258732212160414No Hit
GTTGGCGGATACTCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG240.258732212160414No Hit
GTTGGCGGATACCCTTCCAAACTCTTCTCGACCACATCACCATCCACCTT230.2479517033203967No Hit
GTTGGCGGATACCCTTCCAGACTAGTTTCCTCTTTACTGCAGACTGCGAC220.23717119448037946No Hit
GTTGGCGGATACCCTTCCGTAAAGGCTTTAACACAGAATCAAAAGATTCG210.22639068564036222No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCTTCCTGTGTTGGCGG200.21561017680034494No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACGGAATCAAAAGATTCG180.19404915912031048No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAGTCAAAAGATTCG180.19404915912031048No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAGGATTCG170.18326865028029324No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC150.16170763260025875Clontech Universal Primer Mix Long (96% over 26bp)
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC150.16170763260025875No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCGAAAGATTCG140.1509271237602415No Hit
GTTGGCGGATATCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG140.1509271237602415No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACGCAGAATCAAAAGATTCG140.1509271237602415No Hit
GTTGGCGGATACCCTTCCATAAGGGTGAGCATAGATGTGGCAAGATGGCA140.1509271237602415No Hit
GTTGGCGGATACCTTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG130.14014661492022423No Hit
GTTGGCGGATACCCTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA130.14014661492022423No Hit
GTTGGCGGATACCCTTCCATAAGGGCTTTAACACAGAATCAAAAGATTCG120.129366106080207No Hit
GTTGGCGGATACCCTCCCATAAAGGCTTTAACACAGAATCAAAAGATTCG120.129366106080207No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAGCACAGAATCAAAAGATTCG120.129366106080207No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTGGACAGGAT110.11858559724018973Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCTTCCATAAAGGCTTT110.11858559724018973No Hit
GTTGGCGGATACCCTTCCATCAATTCCTTTAAGTTTCAGCTTTGCAACCA110.11858559724018973No Hit
GTTGGCGGATACCCTTCCATAAGGGTGAGCAAAGATGTGGCAAGATGGCA110.11858559724018973No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCCATAAAGGCTTTA100.10780508840017247No Hit
GTTGGCGGATACCCTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAA100.10780508840017247No Hit
GTTGGCGGATACCCTTCCATAAAGACTTTAACACAGAATCAAAAGATTCG100.10780508840017247No Hit
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT100.10780508840017247No Hit
GTTGGCGGATACCCTTGGACGAAGGACGAAGATGTGGCAAGATGGCAAAC100.10780508840017247No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGGTGGCGGAT100.10780508840017247No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)