FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15188
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA508833.50013168290756No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA467430.77429549644456No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT262417.276797471688173No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG4452.9299446931788253No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC880.5794047932578351No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA810.5333157756123255No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG720.47405846721095596No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT640.42138530418751646No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT640.42138530418751646No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT620.4082170134316566No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG530.34895970503028706No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG520.3423755596523571No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT510.33579141427442716No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT440.28970239662891756No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT360.23702923360547798No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC340.2238609428496181No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGGA270.1777719252041085No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT260.17118777982617855No Hit
TGCCCTCTCTGCCTTTTCTCCCCAGGGTATTTGGTTTCCCAGTCCACTAT250.16460363444824863No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACTTGAGGA190.12509876218066895No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT190.12509876218066895No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT170.11193047142480905No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT170.11193047142480905No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)