FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8973
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA522258.19681266020284No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT123413.752368215758388No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG5105.683717820127048No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA870.9695753928452023No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT760.8469854006463835No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT760.8469854006463835No Hit
TGCCATCTTGCCACATCTGCACCCGCTGCGGTGAGCCTTGAAGCCTAGGG610.6798172294661763No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG570.6352390504847877No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG570.6352390504847877No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT560.6240945057394406No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA510.5683717820127048No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG490.5460826925220105No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT420.4680708793045804No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT410.4569263345592332No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT390.43463724506853896No Hit
AAGGTGGATGGGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT240.26746907388833163No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT230.25632452914298454No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC200.22289089490694305No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGGA180.20060180541624875No Hit
TGCCATCTTGCCACATCTTCTTCCTCGCACCTGCAAGCAGTGGTATCAAC150.1671681711802073No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGTCACCTGCTTCCGGGCTGA150.1671681711802073No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACTTGAGGA140.15602362643486015No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCACTTGAGGA140.15602362643486015No Hit
AAGGGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT140.15602362643486015No Hit
AAGGTGGAGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT130.14487908168951297No Hit
AAGGTGGATGGTGATGTGGTCCTCAATTTAGCCACCTGCTTCCGGGCTGA130.14487908168951297No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACACAGA120.13373453694416582No Hit
AAGGTGGATGGTGATTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT120.13373453694416582No Hit
AAGGTGGATGGTGATGTGGTCTCCAATTTAGCCACCTGCTTCCGGGCTGA120.13373453694416582No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCCGA110.12258999219881869No Hit
AAGGTGGATGGTGTGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT110.12258999219881869No Hit
TGCCATCTTGCCACATCTTCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGG100.11144544745347153No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACACAGA100.11144544745347153No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT100.11144544745347153No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCCTCCGGGCTGA100.11144544745347153No Hit
AAGGTGGATGGTGATGTGGTGGGGGAGGAGGAAGGCGGGTCCGGAAGGGG90.10030090270812438No Hit
AAGGTGGATGGTGATGTGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT90.10030090270812438No Hit
TGCCATCTTGCCACATCTTCACCCGCTGCGGTGAGCCTTGAAGCCTAGGG90.10030090270812438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)