FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9638
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA714074.08175970118282No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT5836.048972815936916No Hit
TGCCATCTTGCCACATCTTAGCCTATGCGGCTCAGCACGAGTGTCATACC740.7677941481635194No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG720.7470429549699108No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG660.6847893753890849No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT300.3112678979041295No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG230.23863872172649928Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAA220.22826312512969496Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC210.21788752853289067No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT210.21788752853289067No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA190.19713633533928202No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA190.19713633533928202No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTACTTGGTTTCCCAGTCCACTA180.1867607387424777No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGCAAGGGT160.16600954554886907No Hit
TGCCATCTTGCCACATCTGAAGGCCGAAGTGGAGAAGGGTATCCGCCAAC150.15563394895206475No Hit
GCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT140.1452583523552604No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGCCCACTA130.13488275575845612No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT130.13488275575845612No Hit
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG130.13488275575845612Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT120.12450715916165178No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTA120.12450715916165178Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTCGGTTTCCCAGTCCACTA120.12450715916165178No Hit
TGCCATCTTGCCACATCTGAAGGCCGAAGTGGGGAAGGGTATCCGCCAAC120.12450715916165178No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC120.12450715916165178No Hit
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGG120.12450715916165178Clontech Universal Primer Mix Long (96% over 27bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACCA110.11413156256484748No Hit
TGCCATCTTGCCACATCTTCAAACATGTAAAATCATCCTCATAGTCCATG110.11413156256484748No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCGCTA110.11413156256484748No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGATATTTGGTTTCCCAGTCCACTA110.11413156256484748No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGAGTATTTGGTTTCCCAGTCCACTA100.10375596596804315No Hit
TGCCCTCTCTGCCTTTTCTCCCCAGGGTATTTGGTTTCCCAGTCCACTAT100.10375596596804315No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)