FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8555
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC238127.831677381648156No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC174220.362361192285213No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT3744.371712448860316No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG2262.641729982466394No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2132.4897720631209816Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTGATGTGGCA1331.554646405610754Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT610.7130333138515488No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC600.701344243132671No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA540.6312098188194039No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT510.5961426066627703No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC480.5610753945061367No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGGTGGCGGAT390.4558737580362361No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT360.4208065458796026No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT360.4208065458796026No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGATGGCGGAT350.4091174751607247No Hit
GTTGGCGGATACCCTTCCATAGGCTTTAACACAGAATCAAAAGATTCGAT330.38573933372296904No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCATGTACTCTGCGTTG320.3740502630040912No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTG240.2805376972530684Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC240.2805376972530684Clontech SMART CDS Primer II A (96% over 26bp)
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA230.26884862653419056No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCTTCCTGTGTTGGCGG220.2571595558153127No Hit
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCACTGCTTCCCATGTAC220.2571595558153127Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT210.24547048509643482Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTCTCCACTTCCCCATGTACTCTGCGTTGATACCAC210.24547048509643482Clontech SMART CDS Primer II A (95% over 21bp)
GTTGGCGGATACCCTTCCATGTTGGCGGATACCCTTCTGTGTTGGCGGAT200.23378141437755698No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT170.19871420222092342No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT170.19871420222092342No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA160.1870251315020456No Hit
GTTGGCGGATACCCTTGCTGAGCCAGCATTAGCAGCTGGTACAGATGTGG160.1870251315020456No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT150.17533606078316774Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTGATGGCACAAGATGTGGCAAGATGGCAAACTGGT140.16364699006428987No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT140.16364699006428987No Hit
GTTGGCGGATACCCTTGCCTGTGTTGGCGGATACCCATGTACTCTGCGTT140.16364699006428987No Hit
GTTGGCGGATACCCTTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT130.15195791934541203Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCCCATGTACTCTGCGTT130.15195791934541203No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT130.15195791934541203No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACCCCATGTACTCTGC130.15195791934541203Clontech SMART CDS Primer II A (95% over 21bp)
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCACCCCATGTACTCTGC130.15195791934541203Clontech SMART CDS Primer II A (95% over 21bp)
GTTGGCGGATACCCTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAA120.1402688486265342No Hit
GTTGGCGGATACCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCA120.1402688486265342No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT120.1402688486265342No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT110.12857977790765635No Hit
GTTGGCGGATACCCTTCCATCAATTCCTTTAAGTTTCAGCTTTGCAACCA100.11689070718877849No Hit
GTTGGCGGATACCCTTGGACATCATCCCATGTACTCTGCGTTGATACCAC100.11689070718877849Clontech SMART CDS Primer II A (95% over 21bp)
GTTGGCGGATACCCTTGCTGTGTTGGCGGATACCCTTGCTGTGTTGGCGG100.11689070718877849No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC100.11689070718877849No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT100.11689070718877849No Hit
CAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT90.10520163646990065No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC90.10520163646990065No Hit
GTTGGCGGATACCCTTCCAAAGGCTTTAACACAGAATCAAAAGATTCGAT90.10520163646990065No Hit
GTTGGCGGATACCCTTCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTT90.10520163646990065No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)