FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23087
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT725031.40295404340105No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT512422.194308485294755No Hit
TGCCATCTTGCCACATCTGTGTTGGACATCATCAAGCAGTGGTATCAACG14286.1852990860657515No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAA12745.518257027764543Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC9524.123532724043835No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA9514.119201282106813No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTA5922.564213626716334Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2931.2691124875471045Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2661.1521635552475418No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGAA1990.8619569454671461Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1880.8143110841599169No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGTA1790.7753281067267294Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGTAT1610.6973621518603543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGAAG1430.6193961969939793Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAT1070.4634642872612293Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTTGTTGGACATCATCAAGCAGTGGTATCAACG1030.446138519513146No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGGT750.3248581452765626Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGTTGGACATCATCAAGCAGTGGTATCAACG720.31186381946550007No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAG530.2295664226621042Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT530.2295664226621042No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT520.22523498072508338No Hit
TGCCATCTTGCCACATCTCCTAAACCCCTGCCCTCGCCTAAGCCTTCTGC490.2122406549140209No Hit
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGAAG490.2122406549140209Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC440.1905834452289167No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT430.18625200329189587No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA350.1516004677957292No Hit
TGCCATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGAAGC350.1516004677957292Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGG330.14293758392168754Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGCAAGGGT330.14293758392168754No Hit
TGCCATCTTGCCACATCTTCTAAACCCCTGCCCTCGCCTAAGCCTTCTGC310.13427470004764586No Hit
TTCTGCGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT280.12128037423658336No Hit
TGCCATCTTGCCACATCTTAGCAAGGGTATCCGCCAACTTGAGGAGACCA270.11694893229956252No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTG270.11694893229956252Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT270.11694893229956252No Hit
TGCCATCTTGCCACATCCAAGCAGTGGTATCAACGCAGAGTACATGGGAA260.11261749036254169Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT240.10395460648850002No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACAAGGGTA240.10395460648850002Clontech SMARTer II A Oligonucleotide (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)