FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22891
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA954241.68450482722467No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC646828.25564632388275No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG16657.2736009785505225No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC8183.573456817089686No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2331.0178672840854486No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1610.7033331877156961No Hit
GTTGGCGGATACCCTTCCACACACGCAAAATACTCCTTCAGCGGAGCGAA1030.4499584989733956No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT970.42374732427591627No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG840.366956445764711No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG740.3232711546022454No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG550.2402691013935608No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG530.23153204316106765No Hit
GTTGGCGGATACCCTTCCATACACGCAAAATACTCCTTCAGCGGAGCGAA530.23153204316106765No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG490.21405792669608142No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG450.1965838102310952No Hit
TACCTCAGTTTGCCCCCTTTCCCCTTTTTTGGCGTCCTAGTAAGATGTGG420.1834782228823555No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA390.17037263553361584No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA380.16600410641736926No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG380.16600410641736926No Hit
TACCTCAGTTTGCCCCTTTCCCCTTTTTTGGCGTCCTAGTAAGATGTGGC370.1616355773011227No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG360.15726704818487616No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA360.15726704818487616No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT320.1397929317198899No Hit
TACCTCAGTTTGCCCCCCCTTTTTTGGCGTCCTAGTAAGATGTGGCAAGA290.12668734437115023No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC290.12668734437115023No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA290.12668734437115023No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA270.1179502861386571No Hit
TACCTCAGTTTGCCCCCCTTTTTTGGCGTCCTAGTAAGATGTGGCAAGAT270.1179502861386571No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA270.1179502861386571No Hit
TACCTCAGTTTGCCCCCTTTTTTGGCGTCCTAGTAAGATGTGGCAAGATG250.10921322790616399No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA230.10047616967367087No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)