FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26674
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA841631.55132338606883No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT788629.564369798305467No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA399414.973382319862038No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC12044.513758716353003No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT11894.457524180850267No Hit
TGCCATCTTGCCACATCCAAGCAGTGGTATCAACGCAGAGTACATGGGTA1940.727299992502062Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCCAAGCAGTGGTATCAACGCAGAGTACATGGGAA1630.6110819524630727Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1600.5998350453625253No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1180.4423783459548625No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT910.34115618204993625No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG760.2849216465471995No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTAAAATCATTGAACATGGTC570.21369123491039965No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGAGTATTTGGTTTCCCAGTCCACTA520.19494638974282072No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT470.17620154457524181No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTA460.17245257554172602Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT460.17245257554172602No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT420.15745669940766288No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT420.15745669940766288No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGTA400.14995876134063132Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAA400.14995876134063132Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC400.14995876134063132No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGAA390.14620979230711553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT350.13121391617305242No Hit
TGCCATCTTGCCACATCCAAGCAGTGGTATCAACGCAGAGTACATGGGGT290.10872010197195772Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG290.10872010197195772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)